The BIO2.0 team shares a common scientific vision of a fundamental approach aimed at understanding complex systems (of natural or artificial origin), and their mastery with a view to their re-engineering in artificial systems. This objective of adapting the elementary functions of living systems (assembly, organization, catalysis, transformations, transport, autonomy, energy conversion, etc.) allows to use them in a technological framework making it possible to respond to current challenges but also within a fundamental framework, by proposing model systems for the understanding of their physical and chemical functions. The issues concern, for example, the emergence of structures and functions, the de novo construction of living systems, energy and energy conversion systems, or the development of new diagnostic methods and therapeutic applications. Our approach is predominantly interdisciplinary and is based on our complementary skills in the physics and chemistry of soft matter, in biochemistry and analytical chemistry, and in analytical micro-technologies.